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Gene Exp Wnt5b Mm01183986 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wnt5b  (Biorbyt)
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The SCD5 Regulates Adipogenesis Through the <t>WNT5B</t> Signaling Pathway. ( a ) WNT5B interference efficiency 48 h post-transfection. siRNA2 was selected for subsequent experiments ( n = 3). ( b ) β-catenin mRNA expression after WNT5B silencing ( n = 3). ( c ) Protein levels of WNT5B , β-catenin , and phosphorylated β-catenin ( p-β-catenin ) as determined by Western blot 48 h post-transfection ( n = 3). ( d ) Cell proliferation (CCK-8) 48 h post-transfection ( n = 3). ( e ) mRNA expression of differentiation markers at 24 h and 8 d. ( f ) Lipid accumulation by Oil Red O staining at day 8. Quantification at 510 nm after extraction and the OD value represents the quantified intracellular triglyceride (TG) content. All data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Wnt5b, supplied by Biorbyt, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp wnt5b hs01086864 m1  (Thermo Fisher)
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Thermo Fisher gene exp wnt5b hs01086864 m1
The SCD5 Regulates Adipogenesis Through the <t>WNT5B</t> Signaling Pathway. ( a ) WNT5B interference efficiency 48 h post-transfection. siRNA2 was selected for subsequent experiments ( n = 3). ( b ) β-catenin mRNA expression after WNT5B silencing ( n = 3). ( c ) Protein levels of WNT5B , β-catenin , and phosphorylated β-catenin ( p-β-catenin ) as determined by Western blot 48 h post-transfection ( n = 3). ( d ) Cell proliferation (CCK-8) 48 h post-transfection ( n = 3). ( e ) mRNA expression of differentiation markers at 24 h and 8 d. ( f ) Lipid accumulation by Oil Red O staining at day 8. Quantification at 510 nm after extraction and the OD value represents the quantified intracellular triglyceride (TG) content. All data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Gene Exp Wnt5b Hs01086864 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wnt5b  (Proteintech)
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Proteintech wnt5b
The SCD5 Regulates Adipogenesis Through the <t>WNT5B</t> Signaling Pathway. ( a ) WNT5B interference efficiency 48 h post-transfection. siRNA2 was selected for subsequent experiments ( n = 3). ( b ) β-catenin mRNA expression after WNT5B silencing ( n = 3). ( c ) Protein levels of WNT5B , β-catenin , and phosphorylated β-catenin ( p-β-catenin ) as determined by Western blot 48 h post-transfection ( n = 3). ( d ) Cell proliferation (CCK-8) 48 h post-transfection ( n = 3). ( e ) mRNA expression of differentiation markers at 24 h and 8 d. ( f ) Lipid accumulation by Oil Red O staining at day 8. Quantification at 510 nm after extraction and the OD value represents the quantified intracellular triglyceride (TG) content. All data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Wnt5b, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The SCD5 Regulates Adipogenesis Through the WNT5B Signaling Pathway. ( a ) WNT5B interference efficiency 48 h post-transfection. siRNA2 was selected for subsequent experiments ( n = 3). ( b ) β-catenin mRNA expression after WNT5B silencing ( n = 3). ( c ) Protein levels of WNT5B , β-catenin , and phosphorylated β-catenin ( p-β-catenin ) as determined by Western blot 48 h post-transfection ( n = 3). ( d ) Cell proliferation (CCK-8) 48 h post-transfection ( n = 3). ( e ) mRNA expression of differentiation markers at 24 h and 8 d. ( f ) Lipid accumulation by Oil Red O staining at day 8. Quantification at 510 nm after extraction and the OD value represents the quantified intracellular triglyceride (TG) content. All data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Animals : an Open Access Journal from MDPI

Article Title: The SCD5 Gene Modulates Adipogenic Differentiation via the WNT5B Signaling Pathway in Xinjiang Brown Cattle

doi: 10.3390/ani15243547

Figure Lengend Snippet: The SCD5 Regulates Adipogenesis Through the WNT5B Signaling Pathway. ( a ) WNT5B interference efficiency 48 h post-transfection. siRNA2 was selected for subsequent experiments ( n = 3). ( b ) β-catenin mRNA expression after WNT5B silencing ( n = 3). ( c ) Protein levels of WNT5B , β-catenin , and phosphorylated β-catenin ( p-β-catenin ) as determined by Western blot 48 h post-transfection ( n = 3). ( d ) Cell proliferation (CCK-8) 48 h post-transfection ( n = 3). ( e ) mRNA expression of differentiation markers at 24 h and 8 d. ( f ) Lipid accumulation by Oil Red O staining at day 8. Quantification at 510 nm after extraction and the OD value represents the quantified intracellular triglyceride (TG) content. All data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: After blocking, the membrane was incubated overnight at 4 °C with the primary antibody prepared in a specialized dilution buffer: GAPDH (1:5000, Proteintech, Wuhan, China, cat #10494-1-AP), CDK1 (1:2000, Proteintech, Wuhan, China, cat #19532-1-AP), CDK6 (1:1000, Proteintech, Wuhan, China, cat #14052-1-AP), PPARγ (1:1000, Proteintech, Wuhan, China, cat #16643-1-AP), C/EBPα (1:1000, Proteintech, Wuhan, China, cat #29388-1-AP), SCD5 (1:1000, Aviva Systems Biology, San Diego, CA, USA, cat #ARP65045_P050), WNT5B (1:1000, biorbyt, Cambridge, UK, cat #orb577989), β-Catenin (1:1000, Abcam, Cambridge, UK, cat #ab32572), and p-β-Catenin (1:500, Affbiotech, Beijing, China, cat #affbiotech).

Techniques: Transfection, Expressing, Western Blot, CCK-8 Assay, Staining, Extraction